次黃嘌呤磷酸核糖基轉(zhuǎn)移酶1(HPRT1)活性蛋白
Active Hypoxanthine Phosphoribosyltransferase 1 (HPRT1)
HGPRT; HGPRTase; HPRT; Lesch-Nyhan Syndrome; Hypoxanthine-guanine phosphoribosyltransferase
- 編號(hào)UAPA717Hu01
- 物種Homo sapiens (Human，人) 相同的名稱(chēng)，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)5.6
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 暫無(wú)報(bào)價(jià) 暫無(wú)報(bào)價(jià) 暫無(wú)報(bào)價(jià) 暫無(wú)報(bào)價(jià) 暫無(wú)報(bào)價(jià)
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) is an enzyme central to the purine salvage pathway. It utilizes phosphoribosylpyrophosphate (PRPP) to transform hypoxanthine into inosine monophosphate (IMP) and guanine into guanosine monophosphate (GMP). APRT, on the other hand, catalyzes the conversion of adenine to adenosine monophosphate (AMP) also with PRPP. When HPRT1 and APRT interact, they can potentially co - ordinate the utilization of PRPP.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human HPRT1 and recombinant human APRT. Briefly, HPRT1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to APRT-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-HPRT1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human HPRT1 and recombinant human APRT was shown in Figure 1, the EC50?for this effect is 0.092ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA717Hu01 | 次黃嘌呤磷酸核糖基轉(zhuǎn)移酶1(HPRT1)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA717Hu01 | 次黃嘌呤磷酸核糖基轉(zhuǎn)移酶1(HPRT1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA717Hu01 | 次黃嘌呤磷酸核糖基轉(zhuǎn)移酶1(HPRT1)多克隆抗體 | WB; IHC; FCM |
| UFAA717Hu01 | 抗次黃嘌呤磷酸核糖基轉(zhuǎn)移酶1(HPRT1)多克隆抗體 | FCM |
| UMAA717Hu22 | 次黃嘌呤磷酸核糖基轉(zhuǎn)移酶1(HPRT1)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA717Hu | 次黃嘌呤磷酸核糖基轉(zhuǎn)移酶1(HPRT1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA717Hu | 次黃嘌呤磷酸核糖基轉(zhuǎn)移酶1(HPRT1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| J Crohns Colitis. | Response to infliximab therapy in ulcerative colitis is associated with decreased monocyte activation, reduced CCL2 expression and downregulation of Tenascin C. [Pubmed:25518051] |
| J Crohns | Response to infliximab therapy in ulcerative colitis is associated with decreased monocyte activation, reduced CCL2 expression and downregulation of tenascin C [PubMed: 25518051] |
| Molecular Biology Reports | Increased ENT2 Expression and Its Association With Altered Purine Metabolism in Different Stages of Colorectal Cancer Cell Lines [] |
