C反應(yīng)蛋白(CRP)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for C Reactive Protein (CRP) ,etc. by FLIA (Flow Luminescence Immunoassay)
C-RP; PTX1; Pentraxin-Related; Pentraxin 1
（注：單次混測多因子不超過8個指標(biāo) ）
- 編號ULMA821Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時長3.5h
- 檢測范圍0.05-50ng/mL
- 靈敏度最小可檢測劑量小于等于0.017 ng/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測C反應(yīng)蛋白(CRP)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的C反應(yīng)蛋白(CRP)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 78-102 | 81 |
| EDTA plasma(n=5) | 94-105 | 99 |
| heparin plasma(n=5) | 91-99 | 96 |
| sodium citrate plasma(n=5) | 87-101 | 97 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的C反應(yīng)蛋白(CRP)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中C反應(yīng)蛋白(CRP)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-101% | 95-105% | 96-104% | 92-99% |
| EDTA plasma(n=5) | 87-101% | 87-101% | 86-93% | 88-96% |
| heparin plasma(n=5) | 84-102% | 78-101% | 86-95% | 88-95% |
| sodium citrate plasma(n=5) | 82-95% | 82-90% | 91-101% | 78-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將C反應(yīng)蛋白(CRP)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的C反應(yīng)蛋白(CRP)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的C反應(yīng)蛋白(CRP)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的C反應(yīng)蛋白(CRP)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
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| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UEPA821Ra61 | C反應(yīng)蛋白(CRP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA821Ra01 | C反應(yīng)蛋白(CRP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA821Ra01 | C反應(yīng)蛋白(CRP)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA821Ra06 | C反應(yīng)蛋白(CRP)多克隆抗體 | WB |
| UPAA821Ra01 | C反應(yīng)蛋白(CRP)多克隆抗體 | WB; IHC |
| ULAA821Ra71 | C反應(yīng)蛋白(CRP)多克隆抗體(生物素標(biāo)記) | WB |
| ULAA821Ra81 | C反應(yīng)蛋白(CRP)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| UMAA821Ra21 | C反應(yīng)蛋白(CRP)單克隆抗體 | IHC |
| UMAA821Ra22 | C反應(yīng)蛋白(CRP)單克隆抗體 | IHC |
| ULAA821Ra82 | C反應(yīng)蛋白(CRP)單克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| ULAA821Ra72 | C反應(yīng)蛋白(CRP)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| USEA821Ra | C反應(yīng)蛋白(CRP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UHEA821Ra | C反應(yīng)蛋白(CRP)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA821Ra | C反應(yīng)蛋白(CRP)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA821Ra | C反應(yīng)蛋白(CRP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA821Ra01 | C反應(yīng)蛋白(CRP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
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| Scientific Reports | Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats [Pubmed: 32616735] |
| animal reproduction science | Quantifying the C-reactive protein concentrations of uterine lavage samples in postpartum dairy cows [Pubmed: 32408965] |
| ANNALS OF TRANSLATIONAL MEDICINE | Serum levels of olfactomedin 4: a biomarker for asthma control state in asthmatics [Pubmed: 32395538] |
| nutrition | Validation and adaptation of the Empirical Dietary Inflammatory Pattern (EDIP) across nations: a test case. [Pubmed: 32563770] |
| European Journal of Biology | Effects of Tempol in Lipopolysaccharide-Induced Liver Injury [] |
| Possible Role of Statins on the Inflammatory Biomarkers in Patients With Periodontal Disease: A Cross-Sectional Study [] | |
| International Journal of Oral Science | Endothelin-1 is a surrogate biomarker link severe periodontitis and endothelial dysfunction in hypertensive patients: The potential nexus [] |
| Indian Journal of Animal Research | Effect of claw disorders on haemato-biochemical parameters and acute phase protein levels in crossbred cattle [] |
| Lateral Flow Immunosensor for Ferritin Based on Dual Signal-Amplified Strategy by Rhodium Nanoparticles [] | |
| Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes [] | |
| МАРКЕРЫ КОСТНОГО РЕМОДЕЛИРОВАНИЯ ПРИ ЗАМЕЩЕНИИ ДЕФЕКТАТРАБЕКУЛЯРНОЙ КОСТНОЙ ТКАНИ РЕЗОРБИРУЕМЫМИ И?… [] | |
| J Transl Med | Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans [33407555] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Use of Fourier-Transform Infrared Spectroscopy (FT-IR) for Monitoring Experimental Helicobacter pylori Infection and Related Inflammatory Response in Guinea Pig??- [33396581] |
| Sci Rep | Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats [33833309] |
| Mol Med Rep | Baicalin relieves Mycoplasma pneumoniae infection?induced lung injury through regulating microRNA?221 to inhibit the TLR4/NF?|êB signaling pathway [34109422] |
| Nutrients | Prevalence and Factors Associated with Iron Deficiency and Anemia among Residents of Urban Areas of S?o Paulo, Brazil [34072813] |
| peerj | Perfluorooctane sulfonate exerts inflammatory bowel disease-like intestinal injury in rats [33510972] |
| Biomed Pharmacother | Intravenous ferric carboxymaltose does not provide benefits in reperfused acute myocardial infarction in the rat with normal iron status [34243097] |
| Aging | Pim-2 kinase inhibits inflammation by suppressing the mTORC1 pathway in atherosclerosis [34547720] |
| Analytical and Bioanalytical Chemistry | Nets of biotin-derived gold nanoparticles as a label for the C-reactive protein immunoassay [34559243] |
| J Oleo Sci | Cardio-protective and Anti-atherosclerosis Effect of Crocetin on Vitamin D3 and HFD-induced Atherosclerosis in Rats [34615830] |
| Medicine | Early-stage serum Stanniocalcin 1 as a predictor of outcome in patients with aneurysmal subarachnoid hemorrhage [Pubmed:34941085] |
| Talanta | A rapid fluorescent aptasensor for point-of-care detection of C-reactive protein [Pubmed:35714415] |
| Experimental and Therapeutic Medicine | Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosis [Pubmed:35126715] |
| Sensors and Actuators B: Chemical | A Two-step Competition Assay for Visual, Sensitive and Quantitative C-reactive Protein Detection in Low-cost Microfluidic Particle Accumulators [] |
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