基質(zhì)細胞衍生因子1(SDF1)多克隆抗體
Polyclonal Antibody to Stromal Cell Derived Factor 1 (SDF1)
CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-Stimulating Factor; Chemokine C-X-C-Motif Ligand 12
- 編號UPAA122Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPA122Hu01-基質(zhì)細胞衍生因子1(SDF1)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.35mg/mL
- 且適物種Mus musculus (Mouse，小鼠)
- 應(yīng)用WB; IHC; ICC/IF; FCM
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 198 ¥ 462 ¥ 660 ¥ 1650 ¥ 6600
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human SDF1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human SDF1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Esophagus cancer Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human SDF1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human SDF1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
AF488 staining on IF;
Sample: THP1 cell
Primary Ab: 20μg/ml Rabbit Anti-Human SDF1 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Western Blot; Sample: THP1 cell lysate Primary Ab: 1μg/ml Rabbit Anti-Human SDF1 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Lane1: Mouse Heart Tissue; Lane2: Mouse Kidney Tissue.
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Western Blot; Sample: Mouse Kidney lysate
Primary Ab: 1μg/ml Rabbit Anti-Human SDF1 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human MCF7 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human SDF1 Polyclonal Antibody (Catalog PAA122Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對SDF1的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別SDF1。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
For flow cytometry: 20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA122Hu01 | 基質(zhì)細胞衍生因子1(SDF1)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA122Hu02 | 基質(zhì)細胞衍生因子1(SDF1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA122Hu62 | 基質(zhì)細胞衍生因子1(SDF1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA122Hu01 | 基質(zhì)細胞衍生因子1(SDF1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA122Hu02 | 基質(zhì)細胞衍生因子1(SDF1)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPA122Hu63 | 基質(zhì)細胞衍生因子1(SDF1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA122Hu61 | 基質(zhì)細胞衍生因子1(SDF1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA122Hu02 | 基質(zhì)細胞衍生因子1(SDF1)多克隆抗體 | WB |
| UPAA122Hu01 | 基質(zhì)細胞衍生因子1(SDF1)多克隆抗體 | WB; IHC; ICC/IF; FCM |
| ULAA122Hu71 | 基質(zhì)細胞衍生因子1(SDF1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAA122Hu21 | 基質(zhì)細胞衍生因子1(SDF1)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA122Hu23 | 基質(zhì)細胞衍生因子1(SDF1)單克隆抗體 | ICC/IF |
| UMAA122Hu22 | 基質(zhì)細胞衍生因子1(SDF1)單克隆抗體 | ICC/IF |
| USEA122Hu | 基質(zhì)細胞衍生因子1(SDF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA122Hu | 基質(zhì)細胞衍生因子1(SDF1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA122Hu01 | 基質(zhì)細胞衍生因子1(SDF1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
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| Phytomedicine | Tanshinone IIA increases recruitment of bone marrow mesenchymal stem cells to infarct region via up-regulating stromal cell-derived factor-1/CXC chemokine receptor 4 axis in a myocardial ischemia model [ScienceDirect: S0944711310003260] |
| Graefe's Archive for Clinical and Experimental Ophthalmology | Monocyte/macrophages promote vasculogenesis in choroidal neovascularization in mice by stimulating SDF-1 expression in RPE cells [PubMed: 21655971] |
| Molecular Therapy | Pleiotropic beneficial effects of sonic hedgehog gene therapy in an experimental model of peripheral limb ischemia [PubMed: 21224834] |
| Oncogene | Elevated PLGF contributes to small-cell lung cancer brain metastasis [PubMed: 22797069] |
| International Journal of Nephrology | Impairment and Differential Expression of PR3 and MPO on Peripheral Myelomonocytic Cells with Endothelial Properties in Granulomatosis with Polyangiitis [PubMed: 22792461] |
| Rheumatology International | Serum levels of selected chemokines in systemic lupus erythematosus patients [Pubmed: 22461186] |
| Experimental Eye Research | Hyperglycemia promotes vasculogenesis in choroidal neovascularization in diabetic mice by stimulating VEGF and SDF-1 expression in retinal pigment epithelial cells [Pubmed: 24780853] |
| Atherosclerosis | The non-alcoholic fraction of beer increases stromal cell derived factor 1 and the number of circulating endothelial progenitor cells in high cardiovascular risk subjects: A randomized clinical trial [Pubmed:24530788] |
| International Journal of Cardiology | Hypoxic exercise training improves cardiac/muscular hemodynamics and is associated with modulated circulating progenitor cells in sedentary men [Pubmed:24286591] |
| Tissue Engineering Part A | Tissue Inhibitor of Metalloproteinase-1 and -3 Improves Cardiac Function in an Ischemic Cardiomyopathy Model Rat [Pubmed:24814095] |
| Pharmacol Rep.? | Comparison of chemokines (CCL-5 and SDF-1), chemokine receptors (CCR-5 and CXCR-4) and IL-6 levels in patients with different severities of depression [Pubmed:25150002] |
| Journal of Stroke and Cerebrovascular Diseases | Circulating CD133+ CD34+ Progenitor Cells and Plasma Stromal-Derived Factor-1Alpha: Predictive Role in Ischemic Stroke Patients [Pubmed:25444027] |
| Pharmacological Reports | The MCP-1, CCL-5 and SDF-1 chemokines as pro-inflammatory markers in generalized anxiety disorder and personality disorders [Pubmed:25560580] |
| In Vitro Cellular & Developmental Biology - Animal | Stromal cell-derived factor 1 (SDF-1) accelerated skin wound healing by promoting the migration and proliferation of epidermal stem cells [Pubmed:25636237] |
| Pharmacological Reports | Serum concentrations of chemokines (CCL-5 and CXCL-12), chemokine receptors (CCR-5 and CXCR-4), and IL-6 in patients with posttraumatic stress disorder and avoidant personality disorder [PubMed: 26481549] |
| Biochem Biophys Res Commun | Stromal cell-derived factor 1 promoted migration of adipose-derived stem cells to the wounded area in traumatic rats [PubMed: 26392311] |
| Am J Transl Res | Role of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive rats [PubMed: 26396666] |
| Medicine (Baltimore) | A Novel Combination of Calprotectin and CXCL13 for Predicting Malignancy in Patients with Exudative Pleural Effusion [PubMed: 26632727] |
| Pharmacological Reports | A study of chemokines, chemokine receptors and interleukin-6 in patients with panic disorder, personality disorders and their co-morbidity [Pubmed:27208878] |
| Frontiers in Psychiatry | Plasma Chemokines in Patients with Alcohol Use Disorders: Association of CCL11 (Eotaxin-1) with Psychiatric Comorbidity. [pubmed:28149283] |
| European?Journal?of?Applied?Physiology | High-intensity?Interval?training?enhances?mobilization/functionality?of?endothelial?progenitor?cellsand?depressed?shedding?of?vascular?endothelial?cells?undergoing hypoxia. [pubmed:27761657] |
| Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease | AMD3100 treatment attenuates pulmonary angiogenesis by reducing the c-kit (+) cells and its pro-angiogenic activity in CBDL rat lungs [pubmed:29233725] |
| International Journal of?Clinical?and Experimental Pathology | Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagens [ISSN:1936-2625/IJCEP0058424] |
| BioMed Research International. | c-Kit-Positive Adipose Tissue-Derived Mesenchymal Stem Cells Promote the Growth and Angiogenesis of Breast Cancer [pubmed:28573141] |
| Scientific Reports | Bone marrow-derived cells and their conditioned medium induce microvascular repair in uremic rats by stimulation of endogenous repair mechanisms. [pubmed:28842629] |
| Biomaterials | Effects of the fibrous topography-mediated macrophage phenotype transition on the recruitment of mesenchymal stem cells: An in vivo study. [pubmed:29017079] |
| Biomaterials?Science | The effects of Sr-incorporated micro/nano rough titanium surface on rBMSC migration and osteogenic differentiation for rapid osteointegration [Pubmed:29850672] |
| International Journal of Molecular?Medicine | Low?intensity pulsed ultrasound promotes periodontal ligament stem cell migration through TWIST1?mediated SDF?1 expression [Pubmed:29620151] |
| Cardiovascular Drugs and Therapy | β‐blockers interfere with cell homing receptors and regulatory proteins in a model of spontaneously hypertensive rats [Pubmed:29752864] |
| Cytotherapy | Mesenchymal stromal cell secretory factors induce sustained improvements in islet function pre-and post-transplantation [Doi: 10.1016/j.jcyt.2018.07.007] |
| ACS?Applied?Materials?&?Interfaces | Functionalization of Silk Fibroin Electrospun Scaffolds via BMSC Affinity Peptide Grafting through Oxidative Self-Polymerization of Dopamine for Bone Regeneration [Pubmed: 30777748] |
| World?Journal of?Stem?Cells | Stromal cell-derived factor-1α promotes recruitment and differentiation of nucleus pulposus-derived stem cells [Pubmed: 30949297] |
| Stem Cells International | Effects of Stromal Cell-Derived Factor-1α Secreted in Degenerative Intervertebral Disc on Activation and Recruitment of Nucleus Pulposus-Derived Stem Cells [Pubmed: 31827537] |
| Stem Cell Res Ther | Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callus [Pubmed: 32894200] |
| Journal of tissue engineering and regenerative medicine | Enhanced recruitment and hematopoietic reconstitution of bone marrow‐derived mesenchymal stem cells in bone marrow failure by the SDF‐1/CXCR4 [Pubmed: 32633015] |
| Acta Biomater | Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia [33878473] |
| pharmaceuticals | bFGF and SDF-1|á Improve In Vivo Performance of VEGF-Incorporating Small-Diameter Vascular Grafts [33800631] |
| Microb Biotechnol | Combination of an engineered Lactococcus?lactis expressing CXCL12 with light‐emitting diode yellow light as a treatment for scalded skin in mice [34310856] |
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