泛素羧基端酯酶L1(UCHL1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)
PARK5; PGP9.5; Uch-L1; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1
- 編號UKSG945Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.78-50ng/mL
- 可適靈敏度0.25ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對泛素羧基端酯酶L1(UCHL1)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPG945Ra02 | 泛素羧基端酯酶L1(UCHL1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPG945Ra01 | 泛素羧基端酯酶L1(UCHL1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAG945Ra01 | 泛素羧基端酯酶L1(UCHL1)多克隆抗體 | WB; IHC |
| ULAG945Ra71 | 泛素羧基端酯酶L1(UCHL1)多克隆抗體(生物素標記) | WB |
| ULAG945Ra81 | 泛素羧基端酯酶L1(UCHL1)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| UMAG945Ra21 | 泛素羧基端酯酶L1(UCHL1)單克隆抗體 | WB; IHC; ICC; IP. |
| USEG945Ra | 泛素羧基端酯酶L1(UCHL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCG945Ra | 泛素羧基端酯酶L1(UCHL1)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMG945Ra | 泛素羧基端酯酶L1(UCHL1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSG945Ra01 | 泛素羧基端酯酶L1(UCHL1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| J Neurol Sci | Changes of ubiquitin C-terminal hydrolase-L1 levels in serum and urine of patients with white matter lesions [PubMed: 26232084] |
| Journal of the Neurological Sciences | Increased plasma UCH-L1 after aneurysmal subarachnoid hemorrhage is associated with unfavorable neurological outcome [Pubmed:26810533] |
| Ulutas Medical Journal | Serum Carnosine Dipeptidase 1 and Ubiquitin C - Terminal Hydrolase L1 as Markers of Brain Damage in Patients after Carotid Endarterectomy [mnstemps:135] |
| Scientific Reports | Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytes [pubmed:28667290] |
| Intensive?Care?Medicine | Relationships between markers of neurologic and endothelial injury during critical illness and long-term cognitive impairment and disability [Pubmed:29523900] |
| Earth and Environmental Science | Serum concentration of ubiquitin c-terminal hydrolase-L1 in detecting severity of traumatic brain injury [article:10.1088] |
| Peripheral blood biomarkers in aneurysmal subarachnoid hemorrhage [ISBN:978-952-03-0750-9] | |
| Journal of Critical Care | Association of neuronal repair biomarkers with delirium among survivors of critical illness [Pubmed: 31896448] |
| Brain Sciences | BDNF and IL-8, But Not UCHL-1 and IL-11, Are Markers of Brain Injury in Children Caused by Mild Head Trauma [Pubmed: 32987792] |
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